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The effect of 100 nM NECA on ERK1/2 phosphorylation was mimicked by compounds increasing intracellular cAMP, like 1 μM forskolin (A), 100 μM cAMP-AM (“caged cAMP”, B), or the PDE inhibitor Ro20-1724 (C) at 100 μM. Western blots show a representative experiment, the columns represent mean values from n = 6 (A), 4 (B), and 5 (C) independent experiments (* p < 0.001, significantly different from control).
