Fig 4. Effect of the PKA inhibitor H-89 on NECA-mediated reduction of ERK1/2 phosphorylation.

Preincubation (30 min) of MDA-MB-231 cells with 10 μM of the PKA inhibitors H-89 prevented the NECA effect on ERK1/2 phosphorylation. The Western blot shows a representative experiment, the columns represent mean values from n = 7 independent experiments (* p < 0.001, significantly different from control).