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. 2018 Aug 29;13(8):e0203079. doi: 10.1371/journal.pone.0203079

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© 2018 Singh et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (a) Spot assay of Gu4/Gu5 and F2/F5 in presence of Ger (135 μg/mL) and FLC. (b) R6G extracellular efflux showing the extracellular R6G concentrations in overexpressing FLC resistant and sensitive match pairs Gu4/ Gu5. The energy dependent R6G efflux was initiated by adding glucose (2%; indicated by an arrow) and quantified by measuring the absorbance of the supernatant at 527 nm. The values are the mean and standard deviations (indicated by error bars) from three independent experiments. (c) UV spectrophotometric ergosterol profiles of Gu4/Gu5 strains scanned between 220 and 300 nm in presence of Ger (135 μg/mL) as depicted in left panel. The relative percentage of ergosterol content in the presence of Ger (135μg/mL) is presented in right panel.