Table 2. FISH positive cases, corresponding molecular subtypes, RNA ISH and clinical data.
| No. | FISH | RNA ISH | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Receptor/ligand | Tumor type | RAS | PIK3CA | BRAF | Ratio | Average FGF(R) signals (%) | Cells with ≥5 FGF(R) signals (%) | Ampli-fication level | H-score | Result | |
| 1 | FGFR1 | C | WT | -- | -- | 1.8 | 5.7 | 60 | Low | 100 | neg (FGFR1) |
| 2*(36) | FGFR1 | R | WT | -- | -- | 1.2 | 5.5 | 66.7 | Low | 101 310 |
neg (FGFR1) pos (FGFR3) |
| 3 | FGFR1 | R | WT | -- | -- | 3.0 | 4.7 | 56.7 | High | 101 | neg (FGFR1) |
| 4 | FGFR1 | R | Mt | -- | -- | 4.4 | 11.4 | 95 | High | 180 | neg (FGFR1) |
| 5 | FGFR1 | R | WT | -- | -- | 2.1 | 3.2 | 33.3 | High | 101 | neg (FGFR1) |
| 6 | FGFR1 | LM/C | WT | WT | WT | 2.0 | 3.6 | 35.0 | High | 105 | neg (FGFR1) |
| 7*(35) | FGFR1 | LM/R | WT | WT | WT | 2.4 | 4.9 | 48.3 | High | 135 360 |
neg (FGFR1) pos (FGFR3) |
| 8*(37) | FGF3,4,19 | R | WT | -- | -- | 2.9 | 5.1 | 45.0 | High | 310 | pos (FGFR3) |
| 9*(28) | FGF3,4,19 | C | WT | -- | -- | 12.9 | 29.9 | 100 | High | 250 | pos (FGFR1) |
*(X), number of the same case in Table 1 ; M, male; F, female; tumor types: C, primary colonic cancer; R, primary rectal cancer; LM/C, liver metastasis with primary tumor in the colon; LM/R, liver metastasis with primary tumor in the rectum; Mt, mutation; WT, wildtype; neg, negative; pos, positive; --, data not available.