Figure 3. Panc1 holoclone cells exhibit an increased tumorigenic potential in vivo.

Tumorigenic potential of Panc1 holoclone and paraclone cells derived from an HSC-enriched coculture was investigated by intrasplenic inoculation of 10⁴ holoclone or paraclone cells into SCID-beige mice (n = 10 animals/cell type). (A) Representative sonographic images of an animal inoculated with Panc1 holoclone cells on day 32 and 145 post operation (POD). A macroscopic tumor lesion is encircled by red line (right panel). (B) Number of animals with macroscopic tumors in pancreas, liver and lung and (C) microscopic tumors in pancreas, liver and lung detected by immunofluorescence staining of human cytokeratin in formalin-fixed paraffin-embedded tissue sections of resected organs. ^* indicates statistically significant differences (p ≤ 0.05).