Table 2. Mutations detected in PIK3CA, ERBB2, ESR1 and AKT1 genes.

	Primary tumor mutations				Relapse mutations
	PIK3CA	ERBB2	ESR1	AKT1	PIK3CA	ERBB2	ESR1	AKT1
Patient 2	x	x	x	x	x	c.3658G>T, p.Gly1220Cys (G3658T, G1220C)	x	x
Patient 4	c.1624G>A, p.Glu542Lys (G1624A, E542K) c.3130A>C, p.Asn1044His (A3130C, N1044H)	x	x	x	c.1624G>A, p.Glu542Lys (G1624A, E542K)	x	x	x
Patient 5	c.3140A>G, p.His1047Arg (A3140G, H1047R)	x	x	x	c.3140A>G, p.His1047Arg (A3140G, H1047R)	x	x	x
Patient 6	x	c.1067C>A, p.Ala356Asp (C1067A, A356D)	x	x	x	c.1067C>A, p.Ala356Asp (C1067A, A356D)	x	x
Patient 7	x	c.-1C>T c.2246C>T, p.Ser749Phe (C2246T, S749F) c.1816G>A, p.Asp606Asn (G1816A, D606N)	x	x	x	x	x	x
Patient 8	x	x	x	x	x	x	c.382G>A, p.Val128Met (G382A, V128M)	c.117G>T, p.Lys39Asn (G117T, K39N) c.288-7C>T c.656C>T, p.Thr219Ile (C656T, T219I)
Patient 9	c.1624G>A, p.Glu542Lys (G1624A, E542K) c.1035T>A, p.Asn345Lys (T1035A, N345K)	x	x	x	c.1035T>A, p.Asn345Lys (T1035A, N345K)	x	x	c.46G>A, p.Gly16Arg (G46A, G16R)
Patient 10	c.3104C>T, p.Ala1035Val (C3104T, A1035V)	c.3526C>T, p.Gln1176Ter (C3616T, Q1206X) c.1096C>T, p.Gln366Ter (C1186T, Q396X) c.1087C>T, p.Gln363Ter (C1177T, Q393X)	x	c.32G>A, p.Trp11Ter (G32A, W11X)	x	x	x	x
Patient 11	c.1633G>A, p.Glu545Lys (G1633A, E545K)	c.1478C>T, p.Pro493Leu (C1568T, P523L)	x	x	c.1633G>A, p.Glu545Lys (G1633A, E545K)	c.1478C>T, p.Pro493Leu (C1568T, P523L)	x	x
Patient 12	x	x	x	x	c.3140A>G, p.His1047Arg (A3140G, H1047R)	x	x	x
Patient 14	x	x	c.1469T>G, p.Met490Arg (T1469G, M490R)	x	x	c.135+3G>T	c.600G>C,p.Trp200Cys (G600C, W200C) c.1048G>A, p.Ala350Thr (G1048A, A350T) c.1469T>G, p.Met490Arg (T1469G, M490R)	x
Patient 15	x	c.1870A>G, p.Ile624Val (A1960G, I654V)	x	x	x	c.1870A>G, p.Ile624Val (A1960G, I654V)	x	x
Patient 16	c.1030G>A, p.Val344Met (G1030A, V344M) c.3140A>G, p.His1047Arg (A3140G, H1047R)	x	x	x	c.1030G>A, p.Val344Met (G1030A, V344M) c.3140A>G, p.His1047Arg (A3140G, H1047R)	x	c.1370-26C>G	x

PIK3CA “Hotspot” mutations reported in red.