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. 2018 Aug 3;9(60):31620–31636. doi: 10.18632/oncotarget.25824

Figure 4. The Hippo kinases are rarely deleted in sarcomas.

Figure 4

(A) Evaluation of The Cancer Genome Atlas data set revealed less than 1% of sarcomas demonstrate deletions of MST2 and LATS2. (B–D) Fluorescence in situ hybridization (FISH) utilizing fluorescent labeled BAC probes hybridizing to metaphase chromosomes and interphase nuclei (inset). (B) FISH probes for MST1 (20q13) demonstrating two signals in metaphase chromosomes and two signals in the interphase nucleus in the A204 cell line, indicating no deletions of the MST1 gene region are present. (C) FISH probes for MST2 (8q22) demonstrating polyploidy, but no deletions of the MST2 gene region. (D) FISH probes for LATS1 (6q25) demonstrate polyploidy, but no deletions of the LATS1 gene region (E) FISH probes for LATS2 (13q12) demonstrate a normal disomic pattern in both the metaphase chromosomes and the interphase nucleus in the A204 cell line, indicating no deletions of the LATS2 gene region (F) FISH for LATS2 in the RD cell line, demonstrating disomic signal pattern in a polyploid cell. Although a deletion or loss of chromosome 13 is present, two copies of LATS2 are still present. (G) Table summarizing FISH results. No deletions were observed in MST1, MST2, or LATS1. A deletion was noted in RD, however the cell line is polyploid, and two copies of LATS2 were still present.