Figure 6. Pk2 and Vangl2 dynamics at shrinking and growing junctions.

(A) Raw GFP-Pk2 pixel intensities strongly correlate with junction length changes. Inset shows a magnified view of the core of the plot. n = 71 junctions from five embryos across four experiments. (B) Raw GFP-Vangl2 pixel intensities strongly correlate with junction length changes. n = 37 junctions from 2 embryos from two different experiments. (C) GFP-caax displays only a weak correlation with junction length changes. Inset shows a magnified view of the core of the plot. n = 108 junctions from seven embryos across six experiments. Even when normalized against GFP-caax, Pk2 and Vangl2 levels show a strong correlation to junction length changes, as shown in Figure 6—figure supplement 1 Figure 6

Figure 6—figure supplement 1. PCP protein localization is correlated with junctional behavior.

(A) Plot of normalized GFP-Pk2 intensities versus change in junction length (r = −0.49; p<0.001; n = 71). Normalization was performed by subtracting background and expressing the intensity of GFP-Pk2 levels as the ratio to the intensity of co-expressed membrane label. (B) Plot of normalized GFP-Vangl2 intensities versus change in junction length (r = −0.57; p=0.002; n = 37). Normalization was performed as per GFP-Pk2, above.

Figure 6—figure supplement 2. Pk2 is more highly enriched at shrinking mediolateral junctions.

(A) Plot of average Pk2 intensities normalized to the membrane label intensities at two time points against the change in the length of the junction divided by the time in seconds between the two time points that the measurements were taken. All measurements are taken from V-junctions that remained within 30 degrees of the mediolateral axis throughout the duration of analysis. Junctions that were shrinking at a greater rate tended to have higher levels of normalized Pk2 localized as compared to other V-junctions that were not shrinking at a comparable rate. n = 205 intervals from 22 cells of five embryos across five separate experiments.