Significant decreases in ChAT-positive cells were observed in the dorsolateral and dorsomedial segments of the dorsal striatum (dorsolateral striatum, two-way ANOVA main effect of genotype F(3,156)=74.77, p<0.0001, main effect of rostrocaudal section, F(5,156)=10.07, p<0.0001, no interaction F(15,156)=1.204, p=0.273; Dorsomedial striatum main effect of genotype F(3,156)=50.01, p<0.0001, main effect of rostrocaudal section, F(5,156)=41.81, p<0.0001, no interaction F(15,156)=1.646, p=0.067. Post-hoc Tukey’s test was performed for all significant main effects. * represents significant difference between ChAT-CKO mice and all control groups). No significant reductions were observed in the ventral segments of the dorsal striatum (ventrolateral; genotype F(3,156)=2.84, p=0.039 [post-hoc Tukey’s test adjusted p=0.129 or higher for all comparisons], rostrocaudal section F(5,156)=0.479, p=0.79, interaction F(15,156)=0.249, p=0.99. ventromedial; genotype F(3,156)= 2.706, p=0.047 [post-hoc Tukey’s test adjusted p=0.107 or higher for all comparisons], rostrocaudal section F(5,156)=46.28 p<0.0001, interaction F(15,156)=0.672, p=0.80).