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. 2018 Aug 17;7:e36691. doi: 10.7554/eLife.36691

Figure 2. ChAT-CKO mice have significantly fewer brainstem and spinal cord cholinergic neurons.

(A,B) Stereological quantification of ChAT-positive or NeuN-positive neurons in the pedunculopontine nucleus (PPN) of control and ChAT-CKO mice (ChAT; t(14)=4.531, p=0.0005. NeuN; t(14)=0.095, p=0.92). (C,D) Stereological quantification of ChAT-positive or NeuN-positive neurons in the laterdorsal tegmental nucleus (LDT) of control and ChAT-CKO mice (ChAT; t(14)=3.571, p=0.003. NeuN; t(14)=1.934, p=0.073). (E,F) Quantification of the number of ChAT-positive neurons in the cervical spinal cord of control and ChAT-CKO mice (t(6)=3.654, p=0.0107). Scale bars = 100 μm.

Figure 2.

Figure 2—figure supplement 1. Absence of gliosis in the brainstem of ChAT-CKO mice.

Figure 2—figure supplement 1.

Immunohistochemistry of GFAP (glial fibrillary acidic protein; specific to astrocytes) and Iba-1 (ionized calcium-binding adapter molecule 1; specific to microglia) in sagittal sections of control and ChAT-CKO hindbrain demonstrate normal distribution of glia.