Table 4. Antibodies used for immunohistochemistry.
| Level | Antigen | Host | Conjugated | Dilution | Source |
|---|---|---|---|---|---|
| Primary | Choline Acetyltransferase | Goat | - | 1:100 | Millipore AB144P |
| Primary | P75 Neurotrophin Receptor | Goat | - | 1:100 | Santa Cruz sc6188 |
| Primary | NeuN | Rabbit | - | 1:500 | Cell Signaling #12943 |
| Primary | GFAP | Mouse | - | 1:1000 | Cell Signaling #3670P |
| Primary | Iba-1 | Rabbit | - | 1:500 | Wako 019–19741 |
| Primary | TorsinA | Rabbit | - | 1:100 | Abcam ab34540 |
| Secondary | anti-mouse | Donkey | Alexafluor-647 | 1:800 | ThermoFisher A-31571 |
| Secondary | anti-rabbit | Donkey | Alexafluor-488 | 1:800 | ThermoFisher A-21206 |
| Secondary | anti-rabbit | Donkey | Alexafluor-555 | 1:800 | ThermoFisher A-31572 |
| Secondary | anti-goat | Donkey | Alexafluor-555 | 1:800 | ThermoFisher A-21432 |
| Secondary | anti-goat | Donkey | biotin | 1:800 | Jackson Immunoresearch 705-065-003 |