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| Region | Marker | Counting frame (μm) | Grid size (μm) | Guard zone (μm) | Dissector (μm) | Section cut thickness (μm) |
|---|---|---|---|---|---|---|
| Striatum | ChAT | 100 × 100 | 250 × 250 | 1 | 10 | 40 |
| NBM | P75 | 90 × 90 | 200 × 200 | 5 | 30 | 50 |
| MS/VDB | P75 | 90 × 90 | 200 × 200 | 5 | 30 | 50 |
| GP | P75 | 100 × 100 | 140 × 140 | 5 | 30 | 50 |
| PPN and LDT | ChAT | 75 × 75 | 150 × 150 | 5 | 30 | 50 |
| PPN and LDT | NeuN | 40 × 40 | 250 × 250 | 5 | 30 | 50 |
