Fig. 6.

Scn2a haplodeficiency in dorsal telencephalic excitatory but in those in global inhibitory neurons reduced neocortical and hippocampal Nav1.2 expression levels. Western blot analyses of 6-weeks neocortex or hippocampus for Scn2a^fl/+/Emx1-Cre, Scn2a^fl/+/Vgat-Cre and Scn2a^fl/+ controls (N = 3, each genotype). Unpaired t-test, Scn2a^fl/+ vs. Scn2a^fl/+/Emx1-Cre, neocortex: t(4) = 5.91, **P = 0.0041; hippocampus: t(4) = 5.74, **P = 0.0046; Scn2a^fl/+ vs. Scn2a^fl/+/Vgat-Cre, neocortex: t(4) = 1.413, P = 0.2305; hippocampus: t(4) = 0.489, P = 0.6503. Nav1.2 protein was normalized by β-tubulin. Mean Nav1.2 expression levels are represented as percentages relative to the level of Scn2a^fl/+ control littermates (100%). Data represent means ± SEM, **P < 0.01