Figure 2:

PTC tolerance of genes. a, Gene tolerance scores for essential genes and dubious ORFs, shown as a violin plot that displays the individual data points. b, Analysis of conditionally essential genes in yeast tetrads. Each vertical set of four colonies corresponds to the four haploid meiotic products from a diploid yeast strain. Each diploid was heterozygous for a deletion mutation of interest and for an interacting mutation. Haploid colonies carrying the deletion of interest are highlighted in red or blue based on their genotype at the interacting locus. Absence of a visible colony (first five panels) indicates a lethal interaction; small colonies (last panel) indicate an interaction causing poor growth. n = 10 tetrads were examined for the ssy1Δ, ptr3Δ, ssy5Δ, and fur1Δ interactions; n = 6 tetrads were examined for the shr3Δ interaction.