. Author manuscript; available in PMC: 2018 Oct 9.

Published in final edited form as: Nat Genet. 2018 Apr 9;50(4):510–514. doi: 10.1038/s41588-018-0087-y

Table 1:

Assessing the efficiency of edit-directing plasmids. Outcomes of directed mutations at eight loci in nej1Δ cells, as determined by classifying paired-end Illumina reads of PCRs of genomic DNA at each locus.

	Expected edit	Unedited	Mismatch	Indel
ho-G582Stop	98.51%	0.08%	1.40%	0.00%
his2-E308Stop	99.83%	0.07%	0.10%	0.00%
mnd1-V219Stop	99.35%	0.53%	0.12%	0.00%
spo11-F381Stop	95.56%	4.24%	0.19%	0.00%
spo13-P252Stop	99.67%	0.20%	0.13%	0.00%
ste3-P469Stop	99.75%	0.12%	0.13%	0.00%
can1-G121Stop	99.81%	0.06%	0.13%	0.00%
can1-G70Stop	99.80%	0.03%	0.17%	0.00%