Table 1. Anti-Campylobacter activity, tolerance to low pH and bile, hydrophobicity, production of H2O2 and biofilm formation of Lactobacillus isolates.
Species | Strain | Results of agar slab method | Ability to survive at low pH | Ability to surviving in MRS + 2% bile | Ability to growth on MRS + 2% bile (%) | Ability to produce H2O2 | Hydrophobicity (%) | Biofilm formation | Resistance to any antibiotic | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Average inhibition zone of C. jejuni (mm) | Average inhibition zone of C. coli (mm) | pH 1.5 | pH 2.0 | ||||||||||
1 hr | 3 hr | 1 hr | 3 hr | ||||||||||
L. salivarius | 4a | 22.5 ± 2.1 | 21.0 ± 0.9 | + | + | + | + | + | no | – | 100 | +++ | + |
6a | 20.7 ± 1.1 | 18.5 ± 0.7 | + | + | + | + | – | no | + | 60 | – | + | |
6b | 21.7 ± 0.35 | 20.5 ± 1.4 | – | – | + | + | + | no | + | 100 | + | + | |
8b | 19.0 ± 0.0 | 18.5 ± 0.7 | + | + | + | + | + | no | + | 67 | – | + | |
9b | 22.2 ± 0.35 | 20.2 ± 1.1 | + | + | + | + | + | no | ++ | 60 | – | – | |
10a | 21.5 ± 2.1 | 20.0 ± 0.0 | + | + | + | + | + | no | + | 100 | – | + | |
10d | 18.2 ± 0.35 | 18.7 ± 1.1 | + | + | + | + | + | no | + | 100 | – | + | |
24a | 22.0 ± 0.0 | 20.0 ± 1.4 | + | + | + | + | + | no | + | 80 | + | + | |
24b | 22.5 ± 0.7 | 20.5 ± 0.7 | + | + | + | + | + | no | +++ | 100 | – | + | |
27e | 25.0 ± 0.0 | 23.5 ± 2.1 | + | + | + | + | + | 48.3 | ++ | 90 | + | + | |
37b | 23.5 ± 0.7 | 20.0 ± 0.0 | + | + | + | + | + | 6.5 | + | 100 | + | + | |
40a | 23.0 ± 0.0 | 18.5 ± 0.7 | + | + | + | + | + | 55.3 | + | 55 | – | + | |
48a | 23.7 ± 0.35 | 20.5 ± 0.7 | + | + | + | + | + | no | – | 100 | + | + | |
60b | 21 ± 1.4 | 19.2 ± 1.1 | + | + | + | + | + | no | – | 10 | + | + | |
60d | 23.5 ± 0.0 | 19.0 ± 0.0 | + | + | + | + | + | no | ++ | 100 | +++ | – | |
L. johnsonii | 4b | 12.5 ± 0.7 | 13.5 ± 0.7 | + | + | + | + | – | no | +++ | 100 | + | – |
7d | 18.5 ± 0.7 | 16.5 ± 2.1 | + | + | + | + | + | 28.0 | +++ | 87 | ++ | + | |
8f | 14.5 ± 0.7 | 13.5 ± 0.7 | + | + | + | + | + | no | +++ | 100 | + | – | |
9d | 12.2 ± 0.35 | 12.5 ± 0.7 | + | + | + | + | – | no | +++ | 50 | + | – | |
13b | 12.0 ± 0.0 | 12.0 ± 0.0 | + | + | + | + | + | no | +++ | 70 | + | + | |
17c | 12.5 ± 0.7 | 12.5 ± 0.7 | + | + | + | + | + | no | +++ | 80 | + | + | |
25b | 11.7 ± 0.35 | 11.7 ± 0.3 | + | + | + | + | + | no | +++ | 60 | – | + | |
32a2 | 14.0 ± 1.4 | 13.4 ± 0.9 | + | + | + | + | + | no | +++ | 100 | – | + | |
37c | 11.5 ± 0.7 | 12.0 ± 0.0 | + | + | + | + | + | no | +++ | 70 | – | + | |
40b | 11.5 ± 0.7 | 11.5 ± 0.7 | + | + | + | + | + | no | +++ | 87 | + | + | |
44c | 14.0 ± 0.0 | 13.0 ± 0.0 | + | + | + | + | + | no | +++ | 100 | + | + | |
L. crispatus | 1b | 15.0 ± 1.4 | 15.0 ± 0.7 | + | – | + | + | + | 23.0 | ++ | 100 | – | + |
17b | 18.2 ± 0.35 | 15.0 ± 1.4 | + | + | + | + | + | 8.9 | ++ | 80 | – | + | |
44b | 16.0 ± 0.0 | 17.0 ± 1.4 | + | + | + | + | + | no | ++ | 100 | – | + | |
49a | 22.2 ± 0.9 | 22.0 ± 0.8 | + | + | + | + | + | no | + | 100 | – | + | |
49b | 14.0 ± 1.4 | 13.2 ± 1.1 | + | + | + | + | + | 14.9 | +++ | 73 | – | – | |
L. reuteri | 4c | 22.0 ± 0.0 | 19.0 ± 1.4 | + | + | + | + | + | 54.6 | ++ | 100 | ++ | + |
12d | 19.5 ± 0.7 | 18.0 ± 1.4 | + | + | + | + | + | 28.6 | +++ | 73 | + | + | |
14a | 24.5 ± 0.7 | 22.0 ± 1.4 | + | – | + | + | + | 69.0 | + | 89 | ++ | + | |
43a | 19.7 ± 2.5 | 17.2 ± 0.3 | + | + | + | + | + | 16.0 | ++ | 90 | – | + | |
45b | 22.0 ± 0.0 | 19.5 ± 0.7 | + | + | + | + | + | 26.4 | ++ | 100 | +++ | + | |
L. ingluviei | 9e | 14.25 ± 0.5 | 12.4 ± 0.5 | + | – | + | – | + | no | +++ | 100 | – | – |
14e | 12.0 ± 0.0 | 12.0 ± 1.4 | + | + | + | + | + | no | ++ | 100 | – | + | |
18b | 14.0 ± 0.0 | 12.0 ± 0.0 | + | – | + | + | + | no | +++ | 100 | + | + | |
28c | 23.7 ± 0.3 | 21.7 ± 1.2 | + | + | + | + | + | no | +++ | 95 | – | + | |
43d | 22.7 ± 0.3 | 19.25 ± 0.3 | + | + | + | + | + | no | +++ | 100 | – | – | |
L. saerimneri | 7b | 16.5 ± 0.7 | 15.5 ± 0.0 | + | – | + | + | + | no | +++ | 100 | – | + |
24c | 14.2 ± 1.1 | 14.0 ± 1.4 | – | – | + | + | – | no | +++ | 100 | – | + | |
44d | 15.7 ± 0.3 | 13.2 ± 1.1 | + | + | + | + | – | no | +++ | 100 | – | + | |
L. oris | 40g | 16.7 ± 0.5 | 13.7 ± 1.5 | + | + | + | + | + | 90.5 | +++ | 91 | + | + |
50c | 17.9 ± 0.6 | 15.4 ± 1.9 | + | + | + | + | + | 88.4 | +++ | 100 | ++ | – | |
Mean: | 18.3 ± 4.3 | 16.7 ± 3.7 |
Grey highlights indicate strains that meet all basic criteria for probiotics.