Table 2. The primer information used in this study.
| Primer pair | Alignment 5′→3′ | Target gene (location) | Amplicon length (bp) | Tm (°C) | Reference | |
|---|---|---|---|---|---|---|
| ACTB | Forward CAA CCG TGA GAA GAT GAC TCA GA | Feline beta actin | 410 | 54 | Kessler et al., 2009 | |
| Reverse CCC AGA GTC CAT GAC AAT AAC A | ||||||
| MY09/11 | Forward GCM CAG GGW CAT AAY AAT GG | PV L1 (Consensus) | approximately 450 | 55 | Manos et al., 1989 | |
| Reverse CGT CCM ARR GGA WAC TGA TC | ||||||
| CP4/5 | Forward ATG GTA CAR TGG GCA TWT GA | PV E1 (Consensus) | approximately 450 | 49 | Tieben et al., 1994 | |
| Reverse GAG GYT GCA ACC AAA AMT GRC T | ||||||
| FcaPV-2 L1 | Forward CGC AAG GAC AGA ATA ATG GTA TTT GCT | L1 | (7130–7156 nt) | 419 | 58 | This study |
| Reverse AAG ACG ATC CGA GAT GTC AAC AT | (7548–7525 nt) | |||||
| FcaPV-3 L1 | Forward TCT GGT AAT CAG TAT AGG GTG TTC AGA GT | L1 | (5868–5876 nt) | 473 | 59 | This study |
| Reverse ATT TCT AAA GGC ACC CCT GAT TTG TCT T | (6340–6312 nt) | |||||
| FcaPV-4 L1 | Forward CTT TGG TAA CCA GCG ATT CC | L1 | (6571–6590 nt) | 476 | 52 | This study |
| Reverse CAA TCT ATC CTT CAA GTC CAC TAC | (7046–7022 nt) | |||||
The name of the primer pairs, the target region of PV ORF, expected size of the PCR product, annealing temperature (Tm), and the reference information are shown respectively.