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Ethidium bromide-imaged agarose gel (1%) of 100 μM pUC19 and 10 μM of each complex in air (5 μM Tris buffer, pH = 7.5, 50 μM NaCl); lanes 1, 2, 3, 5, 7, 9, 11 were kept in the dark and lanes 4, 6, 8, 10, 12 were irradiated (λirr ≥ 395 nm, 5 min); lane 1, plasmid only; lane 2 plasmid treated with SmaI; lanes 3 and 4, 1; lanes 5 and 6, 3; lanes 7 and 8, 5; lanes 9 and 10, 7; and lanes 11 and 12, 8.
