Anti-K10 monoclonal antibodies induce ischemic tissue loss. Female 10 week old C57BL/6 mice received 50 μg of anti-mouse anti-K10 monoclonal antibody by IV tail vein infusion (“Anti-K10 Monoclonal”, n = 5), or an equal amount of normal mouse immunoglobulin (“Control Serum”, n = 5). Representative images are shown at Days 0 and 15 for each of the 10 mice. None of the Control Serum mice had any clinical manifestations (0/5). In contrast, 5/5 Anti-Mouse K10 recipients (100%, Fisher’s Exact p = 0.008) developed cyanosis and ischemia of the ears, with progressive tissue loss (bilateral, though most prominent at arrows). Results are representative of 3 separate experiments.