Table 1.
Atlantic salmon primer sequences used for real-time PCR.
| Gene | Name | Known function in vertebrates | Accession | F/R | Primer | Anneal (°C) |
|---|---|---|---|---|---|---|
| β-actin | beta-actin | Mediator of internal cell motility and growth | AF012125 | F | ATCCTGACAGAGCGCGGTTACAGT | 60 |
| R | TGCCCATCTCCTGCTCAAAGTCCA | |||||
| ef1α | elongation factor 1 alpha | Mediate recruitment of aminoacyl-tRNA to A-site of 80 S ribosome | DQ834870 | F | CACCACCGGCCATCTGATCTACAA | 60 |
| R | TCAGCAGCCTCCTTCTCGAACTTC | |||||
| rpl1 | RNA polymerase 1 | Transcription of ribosomal RNA | NM_001140826.1 | F | ACTATGGCTGTCGAGAAGGTGCT | 60 |
| R | TGTACTCGAACAGTCGTGGGTCA | |||||
| rpl2 | RNA polymerase 2 | Catalyses transcription of DNA to precursors of mRNA | BT049591.1 | F | TAACGCCTGCCTCTTCACGTTGA | 60 |
| R | ATGAGGGACCTTGTAGCCAGCAA | |||||
| b2m | beta 2-microglobulin | Cell surface protein; essential component for stable surface transport and expression | BT046451.2 | F | TCCCAGACGCCAAGCAG | 60 |
| R | TGTAGGTCTTCAGATTCTTCAGG | |||||
| cat | catalase | Catalyses decomposition of H2O2 to water and oxygen; protection of cells from oxidative damage | BT059457 | F | CCCAAGTCTTCATCCAGAAACG | 60 |
| R | CGTGGGCTCAGTGTTGTTGA | |||||
| gpx1 | glutathione peroxidase 1 | Protection of organism from oxidative damage; H2O2 detoxification | DW5665563 | F | GCCCACCCCTTGTTTGTGTA | 60 |
| R | AGACAGGGCTCCACATGATGA | |||||
| gr | glutathione reductase | Catalyses reduction of glutathione disulphide to the sulfhydryl form of glutathione, critical in resisting oxidative stress | XM014199133.1 | F | CCAGTGATGGCTTTTTTGAACTT | 60 |
| R | CCGGCCCCCACTATGA | |||||
| hsp70 | heat shock protein 70 | Important in protein folding and in protecting cells from stress | BG933934 | F | CCCCTGTCCCTGGGTATTG | 60 |
| R | CACCAGGCTGGTTGTCTGAGT | |||||
| sod1 | superoxide dismutase 1 | 1st line defence against reactive oxygen species (ROS); breakdown of potentially harmful ROS in cells | Q3ZLR1 | F | GACCCCACTCTATCATCGGC | 60 |
| R | AATAACTCCACAGGCCAGGC | |||||
| sod2 | superoxide dismutase 2 | C0H894 | F | CTGGGCTTCGACAAGGAGAG | 60 | |
| R | GCTCACGTTCTCCCAGTTGA | |||||
| saa5 | serum amyloid A-5 | Acute phase protein; Secreted during acute phase of inflammation; integral part of innate immune response |
B9EPA2 | F | ACAAGTACTTCCACGCTCGG | 60 |
| R | TCCTCATGTCCTCGACCACT | |||||
| crp/sap1a | C-reactive protein (CRP)/serum amyloid P 1a | Acute phase protein; Binds to molecules on dead/dying cells and activates the complement system | P79905 | F | GGGAGCGTCACTGGATTTCA | 60 |
| R | AGAATCCTCCGTGCACTTCG | |||||
| crp/sap1b | C-reactive protein (CRP)/serum amyloid P 1 b | B5X672 | F | GTGGATGGAGAAGCTGCTGT | 60 | |
| R | GCTTGTCTCGACTGGGATGA | |||||
| il1β | interleukin 1 beta | Cytokine; important mediator of inflammatory response and involved in cell proliferation | Q6IWH5 | F | TGAAGTCCATCAGCCAGCAG | 60 |
| R | GGATGGTGAAGGTGGTGAGG |