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. 2018 Aug 29;8:13025. doi: 10.1038/s41598-018-31328-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NEXN behaves like an SMC differentiation marker in vivo. Panel A shows the Log2 expression level of NEXN in normal human arteries (Na, N = 10) and carotid plaques (Cp, N = 127), respectively (data from the Bike database, see Materials and Methods). Panels B and C show expression of Nexn, Myocd and Srf (B) along with established SMC markers (C) following rat bladder denervation (N = 6 sham-operated control bladders, white bars, and N = 6 denervated bladders, black bars, see Materials and Methods for detail). Panel D shows that Myocd and Nexn correlate in the bladder dataset (P-value and Spearman Rho coefficient are given in graph).