Fig. 7.

Increased Gdh expression suppresses the shopper mutant phenotype. a Locomotion trajectories of third-instar larvae mutant for shopper (shop^C15) expressing Gdh (Gdh), either by presence of the ubiquitous driver (da-Gal4), an ensheathing glia-specific driver (R83E12-Gal4), or a cortex glia-specific driver (R55B12-Gal4) (on the right) are compared to controls that contain the mutation as well as drivers but no Gdh expression transgene (w¹¹¹⁸) (on the left). Expression of Gdh is mediated by an UAS-carrying P-element insertion into the Gdh locus. Upon ubiquitous and ensheathing glial cell-specific expression of Gdh the locomotion is normalized, while no rescue of the locomotion phenotype associated with the shopper^C15 mutant is seen upon expression in cortex glia. b Quantification of peristalsis frequency and peristalsis efficacy. The peristalsis frequency is elevated when Gdh is expressed ubiquitously, whereas the peristalsis efficacy can only be slightly elevated from expression in all cell types. c Quantification of bending probability. Note the rescue of the bending probability upon ubiquitous and ensheathing glial expression of Gdh (the red line indicates the shop^C15 mutants, the blue line indicates the shop^C15 mutants expressing Gdh). Upon expression of Gdh in astrocytes, no rescue is noted and bending probability in both genotypes is identical. Box plots represent the first and third quartile, as well as the median. Whiskers contain values within 1.5 times of the interquartile range, outliers are marked as red crosses, *p < 0.05, ****p < 0.001, n.s.: not significant, Wilcoxon rank-sum test. Due to space limitation please find detailed statistical data in Supplementary Table 1