Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 29;8:13026. doi: 10.1038/s41598-018-31514-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HGF co-treatment prevents dedifferentiation of polarized CFBE cells after prolonged exposure to VX-809. Polarized CFBE-Phe508del cells were treated for 15 days with DMSO, HGF (50 ng/ml), VX-809 (3 μM), or VX-770 (0.5 μM), alone or in the indicated combinations. (a) WB analysis of whole cell lysates. Shown are representative images of immunoblots for ZO-1, E-cadherin, CK8, and CK18 together with bar plots of band intensity quantification, normalized to DMSO (means ± SEM) from three independent assays. Histone H2B was used as a loading normalizer in band intensity quantification. (b–d) Immunofluorescence staining of polarized CFBE-Phe508del cells, treated as in (a), were stained with phalloidin-TRITC, DAPI, and either anti-ZO-1/Alexa 488 (b), anti-CK8/Alexa 488 (c), or anti-CK18/Alexa 488 (d), and analysed by confocal microscopy. Shown are overlay images representative of the indicated treatment conditions. White horizontal bars represent 10 μm. ^*p < 0.05.