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. 2018 Aug 29;8:13035. doi: 10.1038/s41598-018-30811-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Promoter activation analysis in transgenic tadpoles, expressing EGFP under the xRas-dva1 promoter control. (A,A’,A”) The transmitted light (A) and fluorescent (A’) images of whole transgene tadpole on stage 46 at 3 dpa, showing strong activity of xRas-dva1 promoter in the notochord, gut and the head. The enlarged fluorescent image of head region (A”) demonstrates the EGFP fluorescence in ba - branchial arches, ep- epiphysis, gb - gall-bladder, hyp – hypophysis, nc – notochord, op - olfactory pits, st – stomach. (B–E) The transmitted light and fluorescent images of proRas-dva1-EGFP tadpole’s st.42 tail tip just after amputation 0 dpa (B), at 1 dpa (C), at 2 dpa (D) and its sagittal section (E). (F–H) The transmitted light and fluorescent images of proRas-dva1-EGFP tadpole st.52 hindlimb bud at 1 dpa (F), at 2 dpa (G) and its sagittal cryosection (H). Red dashed line indicates the amputation level. The yellow arrowheads point on cells of wound epithelium. The white arrows point on blastema cells. The yellow dashed lines on H indicate wound epithelium borders.