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. 2018 Aug 29;9(9):868. doi: 10.1038/s41419-018-0915-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a and b Rab37 knockdown (shRab37#1 or shRab37#2) promoted sphere-forming ability in H460 lung cancer cells. The representative sphere images (a) and sphere number (b) are shown. Spheres were counted after 7 days of incubation. The sphere number of each group was normalized to the sh-control (shCtrl) group. c RT-qPCR analysis of stemness-related genes in sphere cells derived from shCtrl, shRab37#1 or shRab37#2. Data were normalized to the shCtrl group. d–f Rab37 knockdown enhanced tumor initiation ability in vivo. The xenograft tumors were collected from BALB/c nude mice injected subcutaneously with 500 sphere-derived shCtrl or shRab37#1 H460 cells. The tumor incidence of each group is shown (Upper right) (d). The tumor weight was significantly increased in shRab37 group (e). RT-qPCR analysis of stemness genes (Left) and Rab37 gene (Right) in xenograft tissues derived from H460 cells (f). g and h Rab37^WT or Rab37^Q89L overexpression inhibited lung cancer sphere-forming ability in H1299 cells. The representative sphere images (g) and sphere number (h) are shown. i RT-qPCR analysis of stemness gene expression in H1299 spheres. Data were normalized to vector group. Data are mean ± SEM; N = 3 for cell-based study; N = 6 for animal study. *P < 0.05; **P < 0.01; *** P < 0.001 (Student’s t test)