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. 2018 Aug 29;9(9):872. doi: 10.1038/s41419-018-0853-x

Fig. 1. Expression of α6-integrin in tumor cells derived from human glioblastoma biopsy specimens and downregulation by specific α6-integrin siRNA.

Fig. 1

a α6-Integrin protein expression was analyzed by flow cytometry in tumor cells derived from three human GBM biopsy specimens (GC1, GC2 and GC3) cultured as neurospheres. Images are representative of three independent experiments. b The SFI allowed to evaluate α6-integrin expression level (see Materials and methods section). Results are expressed as the means ± S.E.M. of three independent experiments. c–e Cells were transfected with two different α6-integrin siRNA (si-α6 (1) or si-α6 (2)) or a scramble control (si-Scr). c α6-Integrin mRNA expression was analyzed by real-time PCR. d, e α6-Integrin protein expression was analyzed by flow cytometry. e Quantifications of three experiments are presented as means ± SD. ***p < 0.001;