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. 2018 Aug 29;9(9):872. doi: 10.1038/s41419-018-0853-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cells derived from two GBM biopsy specimens (GC1 and GC2) were transfected with an α6-integrin siRNA (si-α6) or a scramble control (si-Scr). a Different DNA damage response proteins, under basal conditions, or after IR (6 h) were analyzed by western blot. Images are representative of three independent experiments. Actin was used as a loading control. b, c Six hours and 24 h post-irradiation, γH2AX staining was performed and quantified using flow cytometry. Quantifications of three experiments are presented as means ± SD. **0.001 < p < 0.01; *0.01 < p< 0.05