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. 2018 Aug 29;9(9):876. doi: 10.1038/s41419-018-0904-3

Fig. 1. Butein prevents obesity in HFD-fed obese mice.

Fig. 1

a Body weight gain of vehicle control- or butein-treated mice. Male C57BL/6J mice were fed with a low-fat diet (LFD, 10% fat) or high-fat diet (HFD, 60% fat) and treated with vehicle control or butein (5 mg/kg and 15 mg/kg per day) for 8 weeks. Mice were weighed twice per week. b Differences in epididymal fat (eWAT) and liver weight in groups given daily intraperitoneal injection of 5 mg/kg or 15 mg/kg butein. c Representative hematoxylin and eosin (H&E) staining for sections of liver, epididymal fat (eWAT), inguinal fat (iWAT), and brown adipose tissue (BAT) from HFD-fed mice. Scale bar, 50 µm. d Quantification of liver triacylglycerols (TG) levels per protein contents (µg) in HFD-fed mice (n = 5) treated with vehicle control or butein for 8 weeks. e Adipocyte sizes from butein-treated eWAT were smaller than those of control HFD-fed mice. Data represent mean ± s.e.m. Statistically significant differences between control- and butein-treated mice were determined by Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.0005