Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 29;8:13049. doi: 10.1038/s41598-018-31426-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

E202A expression leads to decreased serine/threonine phosphorylation in total cell lysates. (A) Total cell lysates were prepared from WIF-B cells expressing wild type (WT) or the kinase dead STK16 (E202A) after treatment in the absence or presence of 5 μM lactacystin (lac) for 4 h at 37 °C. Lysates (20 μg total protein per lane) were immunoblotted for STK16 (with anti-V5 antibodies) (top panel) or α-tubulin (as a loading control). The asterisk marks a known degradative species of the mutant kinase. Molecular weight standards are indicated on the left (in kDa). (B) The lysates as in A were immunoblotted (IB) with antibodies against phosphorylated-serine/threonine residues (p-ser/thr). Molecular weight standards are indicated on the left (in kDa) and arrows on the right indicate proteins with decreased immunoreactivity in E202A expressing cells. (C) The fold-decrease in immunoreactivity of the proteins marked with arrows in B (from samples without lactacystin) was determined using densitometry. Values represent the average from three independent experiments ± SEM.