Table 1.

Many spots displayed significant changes in immunoreactivity on blots from E202A expressing cells.

Spot (#)	MW (kDa)	Fold-change	Spot (#)	MW (kDa)	Fold-change
1	n/d	−3.3	2	n/d	13.1
4	201.8	−4.3	16	164.1	5.1
25	157.5	−2.4	78	87.5	3.2
32	145.0	−1.9	95	77.5	3.7
52	120.9	−3.0	141	58.3	3.8
57	117.9	−3.2	165	53.4	5.0
73	91.0	−2.4	169	53.3	4.3
81	85.1	−3.7	184	50.1	3.2
92	78.7	−5.5	188	50.1	3.1
101	75.8	−2.8	201	47.5	3.6
112	71.5	−2.6	202	47.5	4.4
117	69.4	−2.1	216	44.7	3.5
123	65.1	−2.4	236	40.4	4.7
127	62.6	−3.1	239	39.6	3.0
172	52.3	−4.0	241	39.5	4.2
247	37.1	−2.1	243	39.3	1.8
276	27.5	−8.5	266	31.5	3.7

The overlapping spots shown in Fig. 3A were analyzed by densitometry (see Materials and Methods). From the 282 spots analyzed, 34 displayed altered immunoreactivity in E202A expressing cells. The spot numbers analyzed (see Fig. 3B) and apparent molecular weights for each are indicated. The fold-change in immunoreactivity is also indicated. The values on the left are those spots with decreased immunoreactivity whereas those on the right, had increased levels. Values represent averages from the technical duplicates.