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. 2018 Aug 29;9(9):881. doi: 10.1038/s41419-018-0898-x

Fig. 2. RASAL2 could modulate RCC angiogenesis.

Fig. 2

a Real-time quantitative PCR and western blot analysis of RASAL2 levels in ACHN cells transfected with RASAL2 shRNAs (KD) and negative control (NC), or 786O cells infected with RASAL2 lentivirus and negative control (NC). GAPDH was used as loading control. b, c Representative pictures and quantification analysis of the recruitment of HUVECs using the conditioned mediums (CMs) collected from ACHN sublines and cocultured system with ACHN sublines. The scale bar is 40 µm. b, d Representative pictures and quantification analysis of the recruitment of HUVECs using CMs collected from 786O sublines and cocultured system with 786O sublines. The scale bar is 40 µm. e MTT assay of HUVECs treated with SFM or diluted CMs from 786O sublines for 48 h. f, g Representative pictures and quantification analysis of HUVECs tube formation treated with serum free medium (SFM) or CMs from ACHN or 786O sublines. *P < 0.05, **P < 0.01, ***P < 0.001