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. 2018 Aug 29;9(9):881. doi: 10.1038/s41419-018-0898-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western blot analysis of c-FOS expression in ACHN or 786O sublines. b Western blot analysis of c-FOS and VEGFA expression in ACHN sublines transfected with c-FOS siRNA. c Western blot analysis of p-GSK3β^S9 and GSK3β in ACHN or 786O sublines. d, e Western blot analysis of c-FOS and VEGFA in ACHN sublines treated with XAV-939 or 786O sublines treated with CT99021. The densitometric analysis of VEGFA expression was shown. f Immunoprecipitation assay of RASAL2 and GSK3β in 786O sublines. GAPDH was used as loading control