Figure 2.

[³H]IAA transport and PIN family expression in root of WT rice seedlings (Shiokari). Seedlings were grown in hydroponic medium containing normal nutrition (control; 2.5 mM N, 300 μM P), low N, or low P (LN, 0.02 mM; LP, 2 µM P), or subjected to treatment with sodium nitroprusside (SNP, 10 µM), 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, 80 µM), or GR24 (analog of SLs, 2.5 µM) for 14 days. (a) [³H]IAA transport; (b) PIN family expression; and (c), OsPIN1b expression. Data are means ± SE of eight replicates (a) and three replicates (b,c). Bars with different letters indicate significant differences at p < 0.05, as determined by ANOVA followed by the LSD test.