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. 2018 Aug 29;8:13014. doi: 10.1038/s41598-018-29784-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OsPIN1b expression and localization of pPIN1b::GUS expression in root tip. Seedlings were grown in hydroponic medium containing normal nutrition (control; 2.5 mM N, 300 μM P), low N, or low P (LN, 0.02 mM; LP, 2 µM ), or subjected to treatment with sodium nitroprusside (SNP, 10 µM), 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, 80 µM), GR24 (analog of SLs, 2.5 µM), or abamine (100 µM) for 14 days. (a–c) OsPIN1b expression under control (a), LN (b), and LP (c) conditions. (d) Localization of pPIN1b::GUS expression. mRNA levels were normalized to that of OsACT. Bar = 1 mm. Data are means ± SE of three replicates and bars with different letters indicate significant differences at p < 0.05, as determined by ANOVA followed by the LSD test.