Figure 4.

Aldehyde detection spectrum (at a concentration of 5 mM) of E. coli host strain CF30 (MG1655 Δsad ΔyqhD) harboring pSENS-20 and pREP-22 as sensing and reporter modules, respectivelyCells were cultivated in M9 mineral medium containing 20 g L⁻¹ glucose and incubated for 12 h with aldehyde inducer when OD₆₀₀ reached ~0.6. SYFP2 fluorescence was calculated using cytometry data based on geometric mean. The reported values represent the mean ± S.D. (n ≥ 2).