Table 2.
Primers used in this study for plasmid construction and strain validation.
| Primer | Sequence (5′-3′) |
|---|---|
| CONSTRUCTION OF SENSING MODULES (pSENS) | |
| CF149 | tatataGtcgagtttacggctagctcagtcctaggtatagtgctagcGGTCCACCGCTTACCCCCCCAAGGGACGAATAAAatgCTACAAAATTGCGCACA |
| CF154 | tgcttaggatcCttaATTCCCCTGCATCG |
| CF161 | taagcactcgagtttatggctagctcagtcctaggtacaatgctagcGGTCCACCGCTTACCCCCCCAAGGGACGAATAAAatgCTACAAAATTGCGCACA |
| CF162 | taagcactcgagctgatggctagctcagtcctagggattatgctagcGGTCCACCGCTTACCCCCCCAAGGGACGAATAAAatgCTACAAAATTGCGCACA |
| CF163 | taagcactcgagCACAGCTAACACCACGTC |
| CF164 | TGTGCGCAATTTTGTAGcatTTTATTCGTCCCTTGGGGGGGTAAGCGGTGGACCAAAGTTAAACAAAATTATTTGTAGAGG |
| CF257 | taagcactcgagtttatggctagctcagtcctaggtacaatgctagcGTCTTAACAAAGGAAAAAATTTACTatgCTACAAAATTGCGCACA |
| CF258 | taagcactcgagtttatggctagctcagtcctaggtacaatgctagcAAATTTACTTATAAAGGAGGAGATAGatgCTACAAAATTGCGCACA |
| CF259 | taagcactcgagtttatggctagctcagtcctaggtacaatgctagcTCGGAAGAAGAATCGAGGAGGAGGTATCAatgCTACAAAATTGCGCACA |
| CONSTRUCTION OF REPORTER MODULES (pREP) | |
| CF155 | gaggccctttcgtcttcacctcgagttaCACATCGGGCAACAGTCC |
| CF156 | gtatttaagttggaaagcttAGGGCAGAGAACGATCTG |
| CF157 | tctctgccctaagctttccaacttaaatacaaggaaaataaggaggtcaacATGGTTAGCAAGGGCGAAG |
| CF158 | gtacgcgtaccatgggatccTTATTATTTATACAGCTCATCCATACCC |
| CF159 | TAAGCActcgagttaCACATCGGGCAACAGTC |
| CF160 | tgcttaAAGCTTTTAAACTTGATCGAGAACGCC |
| CF324 | tgcttaAAGCTTGGTCAGTGCGTCCTGCTGATGTGCTCAGTATCATCGCCAGCGCCCTG |
| CONSTRUCTION OF ALDEHYDE-SYNTHETIC PATHWAY | |
| Pen268 | cggctgctaacaaagcccg |
| Pen269 | gaattctgtgtgaaattgttatccgc |
| Pen321 | tttcacacagaattcGTTTAACTTTAAGAAGGAGATATACCATGGAAGAGAAGCAGATCCTGTGC |
| Pen322 | ctttgttagcagccgggatcctcaTTAATACGTGTAACAGGCCGTAAACAGA |
| STRAIN VERIFICATION | |
| Δsad-ver-fw | CTGCCAGCTTCGGCAA |
| Δsad-ver-rv | GGGTAAAGTCGCGGATTAT |
| ΔyqhD-ver-fw (Pen15) | CAAGCGGCAAATCTCTTCAC |
| ΔyqhD-ver-rv (CF346) | TGGATTAGCCATACGTTCCT |