Table 3.
Constitutive promoters and RBS used for construction of sensing modules in the metabolite sensor.
| Reference | Sequence (5′-3′) | Strength |
|---|---|---|
| PROMOTERS | ||
| BBa_J23106a | TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGC | 1,185 a.u.a |
| BBa_J23114a | TTTATGGCTAGCTCAGTCCTAGGTACAATGCTAGC | 256 a.u.a |
| BBa_J23113a | CTGATGGCTAGCTCAGTCCTAGGGATTATGCTAGC | 21 a.u.a |
| proD, insulated | CACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCT ATGAGTGGTTGCTGGATAACTTTACGGGCATGCATAAGGCTCGTATAATATATT CAGGGAGACCACAACGGTTTCCCTCTACAAATAATT TTGTTTAACTTT |
5,191. a.ub |
| RBSc | ||
| Weak | GGTCCACCGCTTACCCCCCCAAGGGACGAATAAA | 10,000 T.I.R. |
| Very weak | GTCTTAACAAAGGAAAAAATTTACT | 1,000 T.I.R. |
| Medium | AAATTTACTTATAAAGGAGGAGATAG | 100,000 T.I.R. |
| Strong | TCGGAAGAAGAATCGAGGAGGAGGTATCA | 1,000,000 T.I.R. |
a
Nomenclature of promoters and relative promoter strengths as in Registry of Standard Biological Parts (http://parts.igem.org/Part:BBa_J23114).
b
Strength calculated based on information available at Registry of Standard Biological Parts (http://parts.igem.org/Part:BBa_J23114) and study from Davis and co-workers (Davis et al., 2011).
c
RBS with various strengths were designed using online RBS calculator tool (https://salislab.net/software/forward).