FIG 2 .
(A) Growth of ΔpedH, ΔpedH_pedS2S178P, ΔpedH ΔpedS2, and ΔpedH ΔpedR2 strains. The ΔpedH (circles; panel 1), ΔpedH_pedS2S178P (squares; panel 2), ΔpedH ΔpedS2 (diamonds; panel 3), and ΔpedH ΔpedR2 (triangles; panel 4) strains were grown at 30°C and 350 rpm shaking with M9 medium in 96-well plates supplemented with 5 mM 2-phenylethanol in the presence of 10 µM La3+ (blue symbols) or in the absence of La3+ (green symbols). The gray areas in panels 2 to 4 show the time point by which the parental ΔpedH strain (circles) reached an OD600 of >0.4 (dotted line). (B) Activities of the pedE promoter in ΔpedH, ΔpedH_PedS2S178P, ΔpedH ΔpedS2, and ΔpedH ΔpedR2 strains in the presence (blue bars) of 1 µM La3+ or absence of La3+ (green bars) or measured in M9 medium supplemented with 1 mM 2-phenylethanol. Promoter activities are presented in relative light units (RLU × 104) normalized to OD600. All data represent the means for biological triplicates, and error bars correspond to the respective standard deviations.