FIG 4 .

Growth of different P. putida strains at 30°C and 180 rpm shaking with M9 medium in polycarbonate Erlenmeyer flasks supplemented with 5 mM 2-phenylethanol and 10 µM La³⁺ in the absence (A) and presence of kanamycin (B and C) for plasmid maintenance. Flasks were inoculated at an OD₆₀₀ of 0.01 (A) or 0.03 (B and C) with washed cells from M9 overnight cultures grown with succinate in the absence (A) or presence (B and C) of kanamycin and 0.2% (wt/vol) rhamnose to induce pJEM[PedR2] and pJEM[PedR2^D53A] plasmids. (A) Growth of ΔpedE (black circles), ΔpedE_PedS2^S178P (orange circles), and ΔpedE_PedS2^S178P ΔpedR2 (gray circles) strains. (B) Growth of ΔpedH_PedS2^S178P ΔpedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2^D53A] (orange circles). (C) Growth of ΔpedE_PedS2^S178P ΔpedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2^D53A] (orange circles). Data points represent the means for biological triplets, and error bars correspond to the respective standard deviations (positive error values).