TABLE 1 .
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant feature(s) | Reference |
|---|---|---|
| Bacterial strains | ||
| KT2440 | Wild-type strain of Pseudomonas putida (ATCC 47054) | |
| KT2440* | KT2440 with a markerless deletion of upp; parent strain for deletion mutants | 48 |
| ΔpedE | KT2440* with a markerless deletion of pedE | 7 |
| ΔpedE ΔpedS2 | ΔpedE strain with a markerless deletion of pedS2 (PP_2671) | This study |
| ΔpedE ΔpedR2 | ΔpedE strain with a markerless deletion of pedR2 (PP_2672) | This study |
| ΔpedE_PedS2S178P | ΔpedE strain with S178P mutation in PedS2 | This study |
| ΔpedE_PedS2S178P ΔpedR2 | ΔpedE_PedS2S178P strain with a markerless deletion of pedR2 | This study |
| ΔpedH | KT2440* with a markerless deletion of pedH | 7 |
| ΔpedH ΔpedS2 | ΔpedH strain with a markerless deletion of pedS2 | This study |
| ΔpedH ΔpedR2 | ΔpedH strain with a markerless deletion of pedR2 | This study |
| ΔpedH_PedS2S178P | ΔpedH strain with S178P mutation PedS2 | This study |
| ΔpedH_PedS2S178P ΔpedR2 | ΔpedH_PedS2S178P strain with a markerless deletion of pedR2 | This study |
| E. coli BL21(DE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | |
| E. coli DH5α | fhuA2 lac(del)U169 phoA glnV44 φ80′ lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | |
| KT2440*::Tn7-pedE-lux | KT2440* with insertion of miniTn7-pedE-lux | 8 |
| KT2440*::Tn7-pedH-lux | KT2440* with insertion of miniTn7-pedH-lux | 8 |
| ΔpedE::Tn7-pedE-lux | ΔpedE strain with insertion of miniTn7-pedE-lux | 8 |
| ΔpedE::Tn7-pedH-lux | ΔpedE strain with insertion of miniTn7-pedH-lux | 8 |
| ΔpedH::Tn7-pedE-lux | ΔpedH strain with insertion of miniTn7-pedE-lux | 8 |
| ΔpedH::Tn7-pedH-lux | ΔpedH strain with insertion of miniTn7-pedH-lux | 8 |
| ΔpedE ΔpedS2::Tn7-pedH-lux | ΔpedE ΔpedS2 strain with insertion of miniTn7-pedH-lux | This study |
| ΔpedE ΔpedR2::Tn7-pedH-lux | ΔpedE ΔpedR2 strain with insertion of miniTn7-pedH-lux | This study |
| ΔpedE_PedS2S178P::Tn7-pedH-lux | ΔpedE_PedS2S178P strain with insertion of miniTn7-pedH-lux | This study |
| ΔpedH ΔpedS2::Tn7-pedE-lux | ΔpedH ΔpedS2 strain with insertion of miniTn7-pedE-lux | This study |
| ΔpedH ΔpedR2::Tn7-pedE-lux | ΔpedH ΔpedR2 strain with insertion of miniTn7-pedE-lux | This study |
| ΔpedH_PedS2S178P::Tn7-pedE-lux | ΔpedH_PedS2S178P strain with insertion of miniTn7-pedE-lux | This study |
| Plasmids | ||
| pJeM1 | Rhamnose-inducible vector (pBBR1MCS backbone) with low copy number | 51 |
| pJOE6261.2 | Suicide vector for gene deletions | 48 |
| pMW55 | pJOE6261.2-based deletion vector for gene PP_2671 | This study |
| pMW56 | pJOE6261.2-based vector for introducing the S178P mutation in PedS2 | This study |
| pMW61 | pJOE6261.2-based deletion vector for gene PP_2672 | This study |
| pUC18-mini-Tn7T-pedE-lux-Gm | pUC18-mini-Tn7T-Gm-lux vector with the promoter of pedE driving transcription of luxCDABE | 8 |
| pUC18-mini-Tn7T-pedH-lux-Gm | pUC18-mini-Tn7T-lux-Gm vector with the promoter of pedH driving transcription of luxCDABE | 8 |
| pTNS2 | Helper plasmid for Tn7 integration | 49 |
| pJEM[PedR2] | Rhamnose-inducible induction of PedR2 | This study |
| pJEM[PedR2D53A] | Rhamnose-inducible induction of PedR2D53A | This study |