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. 2018 Aug 8;9(8):400. doi: 10.3390/genes9080400

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Nanoparticle tracking analysis of Cryptococcus neoformans extracellular vesicles (EVs) comparing wild type (WT), mutant (graspΔ and atg7Δ) and complemented (graspΔ::GRASP and atg7Δ::ATG7) cells. Results are representative of two independent biological replicates producing similar profiles. Particles were quantified in EV samples suspended in 150 mL phosphate-buffered saline (PBS). Particle detection values shown in the upper, right panel were normalized to the total number of cells in the cultures from which each sample was obtained.