Table 1.
The phagocyte oxidase (PX) domain-containing proteins in Giardia lamblia, Assemble A: phosphoinositide-binding specificities, localization and expression. PIP: phosphoinositide; PVs: peripheral vacuoles; ND: not determined; mRNA: messenger RNA.
| GL50803_16596 | GL50803_42357 | GL50803_16548 | GL50803_24488 | GL50803_16595 | GL50803_7723 | |
|---|---|---|---|---|---|---|
| Basic amino acids | - | - | + | + | + | + |
| PX-domain PIP binding (ordered by affinity) | PI3P | PI3P PI5P PI3,4,5P3 PI3,5P2 PI4,5P2 PI4P |
PI3,4P2 PI3,5P2 PI4,5P2 PI3P PI4P PI3P |
PI3,4,5P3 PI3,4P2 PI3,5P2 PI4,5P2 PI3P PI4P PI3P |
PI3,5P2 PI3P PI4P PI3P PI4,5P2 |
ND |
| Localization in yeast | Endosomes | Endosomes | ND | ND | Cytosolic vesicles Golgi? | Vacuole membrane |
| Localization in G. lamblia | ND | ND | PVs | ND | ND | ND |
| mRNA expression | + | + (mayor expression in cysts) | + | + (mayor expression in cysts) | + | + |