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In situ DPP-IV activity on 2-, 4-, and 6-day Caco-2 cells: (a) using 50 µM Gly-Pro-AMC as a substrate, the fluorescence signal increased as a function of time and was linear at all cell ages over the first 3 min. (b) The fluorescence signal was measured after 3 min as a function of substrate Gly-Pro-AMC concentration at all cell ages. Data are the means ± SD of three experiments performed in triplicate.
