Table 2.

Performances of the metabolic assay depending on the selected threshold. The table shows, for each assayed cut-off level used to define cells as 2-NBDG(high), tumor cell recovery, and number of WBC contaminants. Tumor cell recovery was defined as the number of 2-NBDG(high) Hoechst(+) cancer cells/total number of Hoechst(+) cancer cells × 100. WBC contaminants were defined as the number of white blood cells present in the 2-NBDG(high) population. Cut-off levels were defined as 3-, 5-, and 7-fold the average intensity of the WBC for 2-NBDG as well the average 2-NBDG fluorescence intensity + 2.5-fold its standard deviation (gate “2.5 SD”). Data are presented as average values between all the analyzed spike-in samples.

Cut-Off Level	Tumor Cell Recovery	WBC Contaminants
3-fold WBC average	74.9%	9341
5-fold WBC average	46.5%	3052
7-fold WBC average	32.1%	1412
WBC average + 2.5-folds SD	59.8%	4473