Table 3.
Comparison between the mutations found in the primary tumor and in the 2-NBDG(high) sorted fraction. In bold, we highlighted the mutations detected in the cells that were different or additional with respect to those found in the primary tumor.
| Primary Tumor | 2-NBDG(high) | Matching | |
|---|---|---|---|
| PATIENT 1 | KRAS p.G12C | KRAS p.G12C | Perfect |
| PATIENT 2 | KRAS p.G12C | KRAS p.G12C | Perfect |
| PATIENT 3 | KRAS p.G12C | KRAS p.G12C | Perfect |
| PATIENT 4 * | KRAS p.G12D | KRAS p.G12C | n.a. |
| PATIENT 5 | EGFR p.L858R | EGFR p.L858R | Perfect |
| PATIENT 6 | EGFR p.E746_A750del and EGFR p.T790M | EGFR p.E746_A750del and EGFR p.T790M | Perfect |
| PATIENT 7 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 8 | EGFR p.L858R | EGFR p.L858R | Perfect |
| PATIENT 9 | EGFR p.L858R and EGFR p.T790M | EGFR p.Leu858Arg and EGFR p.T790M | Perfect |
| PATIENT 10 | EGFR p.L858R | EGFR p.L858R | Perfect |
| PATIENT 11 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 12 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 13 | EGFR p.L858R | EGFR p.L858R | Perfect |
| PATIENT 14 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 15 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 16 | EGFR p.E746_A750del | EGFR p.E746_A750del | Perfect |
| PATIENT 17 ** | KRAS exon 2 [not specified] | KRAS p.G12C and KRAS p.G12V | n.a. |
| PATIENT 18 | EGFR p.E746_A750del | EGFR p.T790M | Different |
| PATIENT 19 | KRAS p.G12C | WT | NEG |
| PATIENT 20 | KRAS p.G12C | WT | NEG |
| PATIENT 23 | EGFR p.L858R and EGFR p.T790M | EGFR p.T790M | Partial |
| PATIENT 25 | EGFR p.E746_A750del | EGFR p.T790M | Different |
| PATIENT 26 ** | KRAS p.G12C | KRAS p.G12C and KRAS G12V | Additional |
| PATIENT 27 *** | EGFR p.G719C and EGFR p.T790M | EGFR p.T790M | n.a. |
| PATIENT 29 | KRAS p.G12V | WT | Negative |
| PATIENT 30 | KRAS p.G12C | WT | Negative |
| PATIENT 21 | WT | WT | Perfect |
| PATIENT 22 | WT | EGFR p.E746_A750del | New mutation |
| PATIENT 24 | WT | KRAS p.G12V | New mutation |
| PATIENT 28 | WT | WT | Perfect |
* Patient 4—Like for the other patients, KRAS p.G12C and p.G12V mutations, but not p.G12D, were tested.t. ** Patient 17—The mutational status of the primary tumor was not available in full detail: only the mutated oncogene, without additional details, was present in the records. *** Patient 27—we tested for the EGFR p.T790M mutation, we did not test for EGFR p.G719C. n.a.: not applicable, either due to unavailability of detailed information on primary tumor mutations or unavailability of specific probes.