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. 2018 Jun 12;9(3):202–210. doi: 10.1080/21501203.2018.1482966

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Time-lapse confocal fluorescence images and schematic diagram of intermediate mitosis in an invasive hypha of M. oryzae within the first-invaded rice cell. This strain expresses histone H1-tdTomato and GFP-NLS. The top panel shows five sequential fluorescence pattern stages in both merged bright-field and fluorescence (top), red fluorescence alone (middle), and green fluorescence alone (bottom). The interphase nucleus in G2 appears yellow due to colocalization of H1-tdTomato (red) and GFP-NLS (green) within the nucleus. H1-tdTomato remains associated with DNA throughout the cell cycle. During the early stages of mitosis (M), GFP-NLS spills into the cytoplasm, indicating a change to the integrity of the nuclear envelope. GFP-NLS is contained within the dividing cell by presumed closure of septal pores. Following mitosis, GFP-NLS is reimported back into the nucleus and the nucleus again becomes yellow during interphase (G1). The bottom panel presents a schematic summary of these cellular events. Bar = 5 μm. This figure is modified from Jones et al. (2016a). To view this figure in color, please see the online version of this journal.