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. 2018 Jul 27;10(8):979. doi: 10.3390/nu10080979

Table 1.

Primer sequences used for RT-qPCR.

Gene Primer Direction Primer Sequence
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) Forward 5′-CAAGTTCAACGGCACAGTCAAGG-3′
Reverse 5′-ACATACTCAGCACCAGCATCACC-3′
ATP-binding cassette subfamily G member 5 (ABCG5) Forward 5′-TCTTCCCTGAGCCTAGGGGG-3′
Reverse 5′-CGATTAGCTCTTCCACCCGT-3′
ABCG8 Forward 5′-TTCACGCTCATAGTCGCTGGATAG-3′
Reverse 5′-TGGTTCAATTCTCTTGGACACATCTTC-3′
Cell death-inducing DFFA-like effector A (CIDEA) Forward 5′-TTTCAAACCATGACCGAAGTAGC-3′
Reverse 5′-CCTCCAGCACCAGCGTAACC-3′
Peroxisome proliferator-activated receptor alpha (PPARα) Forward 5′-CCTGAACATCGAGTGTCGAATAT-3′
Reverse 5′-GGTCTTCTTCTGAATCTTGCAGCT-3′
PPAR-gamma coactivator 1alpha (PGC1α) Forward 5′-AAGTGTGGAACTCTCTGGAACTG-3′
Reverse 5′-GGGTTATCTTGGTTGGCTTTATG-3′
PGC1β Forward 5′-GGTCCCTGGCTGACATTCAC-3′
Reverse 5′-GGCACATCGAGGGCAGAG-3′
Sterol regulatory element-binding transcription factor 1a (SREBP1a) Forward 5′-TAGTCCGAAGCCGGGTGGGCGCCGGCGCCAT-3′
Reverse 5′-GATGTCGTTCAAAACCGCTGTGTGTCCAGTTC-3′
SREBP2 Forward 5′-CACAATATCATTGAAAAGCGCTACCGGTCC-3′
Reverse 5′-TTTTTCTGATTGGCCAGCTTCAGCACCATG-3′
Acetyl-CoA carboxylase (ACC) Forward 5′-GCCTCTTCCTGACAAACGAG-3′
Reverse 5′-TGACTGCCGAAACATCTCTG-3′
Fatty acid synthase (FAS) Forward 5′-GCTGCGGAAACTTCAGGAAAT-3′
Reverse 5′-AGAGACGTGTCACTCCTGGACTT-3′
Lipoprotein lipase (LPL) Forward 5′-GACTCGCTCTCAGATGCCCTAC-3′
Reverse 5′-GCCTGGTTGTGTTGCTTGCC-3′
CD antigen 36 (CD36) Forward 5′-TGGTGGATGGTTTCCTAGCCTTTC-3′
Reverse 5′-TCGCCAACTCCCAGGTACAATC-3′
Adrenoreceptor beta 3 (ADRB3) Forward 5′-ACCAACGTGTTCGTGACT-3′
Reverse 5′-ACAGCTAGGTAGCGGTCC-3′
CPT1 Forward 5′-ATCTGGATGGCTATGGTCAAGGTC-3′
Reverse 5′-GTGCTGTCATGCGTTGGAAGTC-3′
CPT2 Forward 5′-GCCTGCTGTTGCGTGACTG-3′
Reverse 5′-TGGTGGGTACGATGCTGTGC-3′
Cytochrome c oxidase subunit 8B (COX8B) Forward 5′-TGTGGGGATCTCAGCCATAGT-3′
Reverse 5′-AGTGGGCTAAGACCCATCCTG-3′
Uncoupling protein 1 (UCP1) Forward 5′-AGATCTTCTCAGCCGGAGTTT-3′
Reverse 5′-CTGTACAGTTTCGGCAATCCT-3′