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. 2018 Aug 15;9(8):413. doi: 10.3390/genes9080413

Figure 9.

Figure 9

Malate dehydrogenase (MDH) and proline dehydrogenase (PRODH) expression and activity in the T. cruzi exponential and stationary phases. (A) LFQ expression of MDH and PRODH. MDH1 and MDH2 are two MDH isoforms associated to the Tc00.1047053506937.10 and Tc00.1047053507883.100 genes. MDH protein expression was only found in the exponential growth phase, while PRODH expression was upregulated in the stationary phase. (B) MDH and PRODH enzymatic activity measured in the exponential (EPE) and stationary (SPE) phases. The MDH was measured in the sense of the reduction of oxaloacetate (OAA) to form malate with the concomitant oxidation of NADH to NAD+. The consumed NADH was followed by spectrophotometric absorbance at 340 nm at constant temperature (37 °C). The PRODH enzyme activity was measured by the reduction of dichlorophenolindophenol (DCPIP) with the concomitant oxidation of L-Pro to 1D-Pyrroline-5-carboxylate (P5C). The reduction of DCPIP was monitored by spectrophotometric absorbance at 600 nm at constant temperature (37 °C). (B) The specific enzymatic activity of MDH and PRODH were measured in epimastigote cell-free extracts in exponential and stationary phase. No statistical difference between exponential and stationary phase were found for MDH and PRODH activities (MDH p-value = 0.5447 and PRODH p-value = 0.0612).