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. 2018 Aug 8;7(8):99. doi: 10.3390/cells7080099

Figure 5.

Figure 5

CEP55 promotes MMP expression via activating JAK2–STAT3 signaling. (A) Huh-7 cells were pre-infected with the indicated CEP55 shRNA for 72 h, and then cells were incubated with or without 20 ng/mL IL-6 for 30 min, followed by an analysis of the expression of the indicated proteins by western blotting; (B) Flag-tagged CEP55 was pre-transfected into Hep3B cells for 72 h, and then cells were incubated with or without 20 ng/mL IL-6 for 30 min, followed by an analysis of the expression of the indicated proteins by western blotting; (C) Vector or Flag-CEP55 plasmids were transfected into HepG2 cells. JAK2 was immunoprecipitated with anti-flag antibody; (D) Vector or flag-CEP55 plasmids were transfected into Hep3B cells. Ectopic CEP55 was immunoprecipitated with anti-JAK2 antibody; (E) Immunoprecipitation of CEP55, JAK2, and IgG in HepG2 cells, and western blotting analysis of the indicated proteins; (F) Flag-tagged CEP55 was pre-transfected into Hep3B cells for 24 h, followed by treatment with the control, JAK2, and STAT3 shRNA for 48 h. Expression levels of the indicated proteins were analyzed by western blotting.