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. 2018 Aug 8;10(8):414. doi: 10.3390/v10080414

Figure 2.

Figure 2

Innate immune responses induced by MVA-HCV and MVA-HCV ΔC6L in human macrophages and monocyte-derived dendritic cells (moDCs). Human THP-1 (human monocytic cell line derived from an acute monocytic leukemia patient) differentiated to macrophages (A) and moDCs (B) were mock infected or infected with MVA-WT, MVA-HCV, or MVA-HCV ΔC6L (5 PFU/cell in panel A and 0.3 or 1 PFU/cell in panel B). At 6 hpi, RNA was extracted and interferon (IFN)-β, IFN-β-induced gene (IFIT)1, IFIT2, tumor necrosis factor alpha (TNF-α), and hypoxanthine-guanine phosphoribosyltransferase (HPRT) mRNA levels were analyzed by RT-PCR. Results are expressed as the ratio of the gene to HPRT mRNA levels. A.U., arbitrary units. p values indicate significant response differences when comparing MVA-HCV and MVA-HCV ΔC6L with MVA-WT (* p < 0.05, ** p < 0.005). Data are means ± SD of duplicate samples from one experiment and are representative of two independent experiments.